why not use moleclar testing, culture is v insensitive and is not the method 
of choice
"HD-NZ" <IsaAvasa@gmail.com> wrote in message 
news:1153298426.808560.49130@i3g2000cwc.googlegroups.com...
> Does any one have a procedure on how to propagate and freeze CMV for
> use as a positive shell vial control?  Some specific questions:
> 1)  Is it better to aliquot the controls so that they are one-time use?
> 
> 2)  Is freezing the specimen better vs. propagating virus in tissue
> culture first - then freezing?
> 3)  Is a 50% Sorbitol combined equally with Hanks Balanced Salt a good
> medium to freeze CMV?
> 
> Thank you!
> H
>